구강편평상피세포암종에서 shikonin에 의한 상피간엽이행 억제
Shikonin inhibits the Epithelial-Mesenchymal Transition in Oral Squamous Cell Carcinoma
박단비, 박봉수, 강해미, 유수빈, 김인령,
소속 상세정보
박단비 ( Park Dan-Bi ) - 부산대학교 치의학전문대학원 구강해부학교실
박봉수 ( Park Bong-Soo ) - 부산대학교 치의학전문대학원 구강해부학교실
강해미 ( Kang Hae-Mi ) - 부산대학교 치의학전문대학원 구강해부학교실
유수빈 ( Yu Su-Bin ) - 부산대학교 치의학전문대학원 구강해부학교실
김인령 ( Kim In-Ryoung ) - 부산대학교 치의학전문대학원 구강해부학교실
KMID : 0355820190400010051
Abstract
Oral cancer invades the surrounding muscles and bone tissue and metastasizes to the cervical lymph nodes. The 5-year survival rate of patients with metastases is about 40%, and it is necessary to develop anticancer drugs that suppress metastasis to improve the prognosis of patients. In epithelial-mesenchymal transition (EMT), the squamous cancer cells have mobility, which increases the invasiveness and metastasis to new organs. Therefore, EMT inhibition is considered a therapeutic method for inhibiting the metastasis of cancer cells. Shikonin has been isolated and purified from Lithospermum erythrorhizon Sieb. et Zucc and is widely known for its anti-inflammatory effect. Recently, shikonin has been shown to induce apoptosis and cell cycle arrest in various cancer cells and to inhibit the metastatic properties of lung and gastric cancer cells. However, the effects of shikonin on oral cancer are still unclear. Therefore, we investigated the effect of EMT inhibition on shikonin-treated oral cancer cell line, CAL-27 cells. Shikonin treatment inhibited the viability and proliferation of CAL-27 cells. In addition, shikonin inhibited their migration and invasion in wound-healing and transwell invasion assays. Shikonin increased the protein expression of E-cadherin, an epithelial marker in CAL-27 cells, and decreased the protein and RNA expression of mesenchymal markers like N-cadherin, Snail, and Slug. Therefore, shikonin inhibited the epithelial-mesenchymal metastasis of oral cancer cell line CAL-27, suggesting that it may be an anticancer agent with an anti-metastatic effect. 줄이기
키워드
Shikonin; EMT; migration; invasion; OSCC
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